* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pEK RS3

$30.00

From Karl Johnson, Haverford College, October 2005

RSP3 genomic sequence as originally described by Williams et al. (“pEK RS3”). A 36 bp insertion was cloned into a Stu I site near the end of the RSP3 coding sequence, such that an HA epitope is encoded near the extreme carboxy-terminus of RSP3 (Kozminski et al, 1993) Digestion with Eco R1 releases the entire genomic+tag insert (~4Kb) from the vector (3 Kb). Transformation of this plasmid into pf14 resulted in restoration of wild-type motility and the presence of HA-tagged RSP3 in flagella.

amp resistant

Williams BD, Velleca MA, Curry AM, Rosenbaum JL (1989) Molecular cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: flagellar mutation pf-14 is an ochre allele. J Cell Biol 109:235-245 Kozminski KG, Diener DR, Rosenbaum JL (1993) High level expression of nonacetylatable alpha-tubulin in Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 25:158-170

pEK RS3

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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