CC-893 ery2d mt-
$30.00
From Lawrence Bogorad, Harvard University, February 1980. This culture replaced one sent from the Culture Centre of Algae and Protozoa (CCAP), in January 1980.
Phenotype: antibiotic resistant (erythromycin)
The ery2d allele was obtained in Bogorad’s laboratory after mutagenesis of a 137c wild type strain with ethyl methanesulfonate..
The ery2 mutants as a group are resistant to 100 micrograms/ml erythromycin on minimal medium, and are cold-sensitive. Please see CC-1711 for more information on the ERY2 locus.
Mets LJ, Bogorad L (1971) Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes. Science 174:707-709
Mets LJ, Bogorad L (1972) Altered chlorplast ribosomal proteins associated with erythromycin-resistant mutants in two genetic systems of Chlamydomonas reinhardi. Proc Natl Acad Sci USA 69:3779-3783
Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89:281-297
Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353