CC-1026 pf3 mt+
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
The original stock was obtained by Luck from Nam-Hai Chua, who got it from R.P. Levine. This stock is derivative pf3.1B+ from Luck’s lab.
Cells of this strain swim slowly.
This mutation appears to affect the dynein regulatory complex. Yanagisawa and Kamiya showed that both pf3 and ida6 are deficient in the tektin homolog p58, but that neither mutant mapped to the same position as this gene, nor did pf3 and ida6 appear to be allelic with one another.
It has been been reported by Susan Dutcher that this copy of CC-1026 pf3 mt+ carries a cnk11-6 mutation. Please see CC-5145 pf3 (wild-type for CNK11) for a pf3 strain with a verified wild type CNK11. -September 2015
Piperno G, Mead K, Shestak W (1992) The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella. J Cell Biol 118:1455-1463
Gardner LC, O'Toole E, Perrone CA, Giddings T, Porter ME (1994) Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella. J Cell Biol 127:1311-1325
Piperno G, Mead K, LeDizet M, Moscatelli A ( 1994) Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes. J Cell Biol 125:1109-1117
Yanagisawa HA, Kamiya R ( 2004) A tektin homologue is decreased in chlamydomonas mutants lacking an axonemal inner-arm dynein. Mol Biol Cell 15:2105-2115
Piperno G, Mead K, Shestak W (1992) The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella. J Cell Biol 118:1455-1463
Gardner LC, O'Toole E, Perrone CA, Giddings T, Porter ME (1994) Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella. J Cell Biol 127:1311-1325
Piperno G, Mead K, LeDizet M, Moscatelli A ( 1994) Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes. J Cell Biol 125:1109-1117
Yanagisawa HA, Kamiya R ( 2004) A tektin homologue is decreased in chlamydomonas mutants lacking an axonemal inner-arm dynein. Mol Biol Cell 15:2105-2115