CC-1030 pf13 mt+
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
This is Luck’s strain pf13.4A+. We aren’t certain whether this is the original pf13 mutant isolated by Lewin, or the allelic mutant pf13a isolated by Huang et al. (please see CC-2492 for an unequivocal pf13a strain).
Mutants at this locus have a defect in the dynein outer arms. Flagella are short in exponentially growing cells but reach full length in stationary phase cultures. Motility is impaired, and it is not difficult to score.
Omran et al. showed that the PF13 locus corresponds to a gene encoding a conserved protein involved in assembly of outer arm dynein.
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Piperno G, Luck DJ (1981) Inner arm dyneins from flagella of Chlamydomonas reinhardtii. Cell 27:331-340
Witman GB, Minervini N (1982b) Role of calmodulin in the flagellar axoneme: effect of phenothiazines on reactivated axonemes of Chlamydomonas. Prog Clin Biol Res 80:199-204
Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskeleton 32:98-102
Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H (2008) Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature 456:611-616
Yamamoto R, Hirono M, Kamiya R (2010) Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins. J Cell Biol 190:65-71
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Piperno G, Luck DJ (1981) Inner arm dyneins from flagella of Chlamydomonas reinhardtii. Cell 27:331-340
Witman GB, Minervini N (1982b) Role of calmodulin in the flagellar axoneme: effect of phenothiazines on reactivated axonemes of Chlamydomonas. Prog Clin Biol Res 80:199-204
Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskeleton 32:98-102
Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H (2008) Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature 456:611-616
Yamamoto R, Hirono M, Kamiya R (2010) Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins. J Cell Biol 190:65-71