* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-1034 pf16 mt+

Name: CC-1034 pf16 mt+
SKU: CC-1034
Availability: InStock

$30.00

From David Luck, Rockefeller University, 1980

Phenotype: impaired motility

Luck’s stock of this mutant was obtained from George Witman, and is presumed to be the original allele isolated by Lewin. This strain is Luck’s derivative pf16.28A+. Two allelic mutants were isolated in Luck’s laboratory, of which one is in our collection (CC-2510). Also see CC-3896 for a temperature-sensitive allele.

This mutation is in a gene encoding a flagellar central apparatus protein. The mutant phenotype is easy to score.

McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099

Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236

Bernstein M, Beech PL, Katz SG, Rosenbaum JL (1994) A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum. J Cell Biol 125:1313-1326

Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99

Smith EF (2002b) Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase. Mol Biol Cell 13:3303-3313

Locus:
PF16
Chromosome:
9

McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099

Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236

Bernstein M, Beech PL, Katz SG, Rosenbaum JL (1994) A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum. J Cell Biol 125:1313-1326

Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99

Smith EF (2002b) Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase. Mol Biol Cell 13:3303-3313

CC-1034 pf16 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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