CC-1312 spr-u-sp-23 sr-u-sm2 mat-1 mt-
$30.00
From Ruth Sager, Sidney Farber Cancer Institute, May 1982
Phenotype: antibiotic resistant (spectinomycin, streptomycin)
Originally described by Sager as a spontaneous mutation in a culture of spc sm2 mt-, mat-1 appeared to increase the frequency of zygotes biparental for chloroplast markers in crosses, and was reported to be closely linked to the mating type locus. Subsequent work by Gillham et al. showed that the original mat-1 strain was a diploid stock rather than a new haploid mutation.
CC-1312 was used by Nishiyama et al. in a study of methylation of chloroplast DNA that resulted in cloning of the chloroplast-resident DNA methyltransferase.
Sager R, Ramanis Z (1974) Mutations that alter the transmission of chloroplast genes in Chlamydomonas. Proc Natl Acad Sci U S A 71:4698-4702
Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Mating type linked mutations which disrupt the uniparental transmission of chloroplast genes in chlamydomonas. Genetics 115:677-684