CC-1391 fla3 mt-
$30.00
From David Luck, Rockefeller University, February 1983
The fla3 mutant shows abnormalities in flagellar assembly at 21 degrees, and loses its flagella at 34 degrees. This is the original fla3 mutant. Iomini et al. reported in 2001 that their stock had lost its phenotype and excluded it from their further analysis. Mueller et al. also found that CC-1391 no longer had a clear temperature-sensitive phenotype, but were able to recover a good isolate from a backcross to 21 gr (CC-1690). One progeny clone from this cross (1B) was designated as fla3-1, and is now the representative allele for this mutation (see CC-4283). Mueller et al. showed that this is a mutation in the nonmotor subunit of Kinesin-2, a component of the anterograde intraflagellar transport complex.
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII. Genetics 100:579-586
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24
Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341-1354