CC-1392 fla4 mt+
$30.00
From David Luck, Rockefeller University, February 1983
Phenotype: impaired motility
The fla4 mutant fails to assemble flagella at 34 degrees. The FLA4 locus was mapped close to FLA3 on chromosome 10, but Mueller et al. found fla4 showed no alterations in the KAP gene, site of the fla3 mutation, and they were unable to rescue fla4 with a BAC clone containing the wild-type KAP gene.
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII. Genetics 100:579-586
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24
Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341-1354