CC-1395 fla10-14 (formerly fla7) mt+
$30.00
From David Luck, Rockefeller University, February 1983, as fla7 isolation # 519.
Phenotype: impaired motility
This mutant was originally mapped in Luck’s laboratory to linkage group IV, based on limited tetrad analysis, but subsequently was found to be an allele of fla10 on linkage group XIX (chromosome 17; see Lux and Dutcher, 1991). Please see CC-1919 for more information on the FLA10 locus).
The original fla7 mutant was isolated in an ery10 background (see CC-70). We don’t know if this strain is resistant to erythromycin.
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII. Genetics 100:579-586
Lux FG 3rd, Dutcher SK (1991) Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii. Genetics 128:549-561
Miller MS, Esparza JM, Lippa AM, Lux FG 3rd, Cole DG, Dutcher SK (2005) Mutant kinesin-2 motor subunits increase chromosome loss. Mol Biol Cell 16:3810-3820
Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896