CC-1397 sup-pf1 mt-
$30.00
From David Luck, Rockefeller University, February 1983, his isolation number pf6R6
This is the original sup-pf1 allele described by Huang et al. as R6, a suppressor of pf6. It was characterized as a mutation in the spoke specific suppressor system, which restores motility to radial spoke- and central-pair defective mutants without altering spoke or central pair defects. It was subsequently shown to be an allele at the ODA4 locus, corresponding to the gene encoding the beta heavy chain of the outer dynein arm.
Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-124
Porter ME, Knott JA, Gardner LC, Mitchell DR, Dutcher SK (1994) Mutations in the SUP-PF-1 locus of Chlamydomonas reinhardtii identify a regulatory domain in the beta-dynein heavy chain. J Cell Biol 126:1495-1507
Sakato M, King SM (2003) Calcium regulates ATP-sensitive microtubule binding by Chlamydomonas outer arm dynein. J Biol Chem 278:43571-43579
Mitchell BF, Grulich LE, Mader MM (2004) Flagellar quiescence in Chlamydomonas: Characterization and defective quiescence in cells carrying sup-pf-1 and sup-pf-2 outer dynein arm mutations. Cell Motil Cytoskeleton 57:186-196