* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pAV3

$30.00

From Adrian Valli, David Baulcombe lab, University of Cambridge, UK, March 2016

This construct expresses the spectinomycin resistance gene (derived from pALM32) under a constitutive promoter. It confers resistance to ampicillin (carbenicillin) in E. coli and resistance to paromomycin in transgenic Chlamydomonas lines. This construct in particular carries an intron in the middle of the spectinomycin resistance gene that lacks the miRNA stem-loop. For further details, please see Valli et al., 2016, Genome Research. In this manuscript the plasmid is under the name of spect/intron.

host strain: DH5α
amp resistant

Sequence

Valli AA, Santos BA, Hnatova S, Bassett AR, Molnar A, Chung BY, Baulcombe DC (2016) Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs. Genome Res. 26:519-29

pAV3

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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