CC-5209 fdx5 mt-
$30.00
From Wenqiang Yang, Grossman lab, Carnegie Institution for Science – Department of Plant Biology, February 2016
The fdx5 mutant is disrupted in the second exon by a cassette conferring resistance to paromomycin. It was generated in the CC-4425 (D66+) WT background and was backcrossed 4 times to either CC-124 (mt-) or CC-125 (mt+).
The fdx5 mutant cannot grow in the dark, so it should be maintained on TAP medium in the light. Very low light (> 5 µmol photons m-2 s-1) is sufficient for growth of the fdx5 mutant. It is also resistant to paromomycin.
FDX5 – Cre17.g700950; g16996
Yang W, Wittkopp TM, Li X, Warakanont J, Dubini A, Kim RG, Nowack ECM, Catalanotti C, Aksoy M, Mackinder LCM, Page MD, D’Adamo S, Heinnickel M, Johnson X, Richaud P, Alric J, Boehm M, Jonikas M, Benning C, Merchant SS, Posewitz MC, Grossman AR (2015) Critical role of Chlamydomonas reinhardtii Ferredoxin-5 in maintaining membrane structure and dark metabolism. Proc. Natl. Acad. Sci. USA. 112:14978-14983.