* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5422 saga1::SAGA1-Venus3xFLAG

Name: CC-5422 saga1::SAGA1-Venus3xFLAG
SKU: CC-5422
Availability: InStock

$30.00

From Alan Itakura, Jonikas lab, Princeton University, January 2019

Cre11.g467712 insertion at Intron 26; saga1 expressing pRAM118-SAGA1-Venus-3xFLAG

SAGA1 was cloned into the pRAM118 vectors and transformed into strain saga1 (CC-5420). Lines were then selected for antibiotic resistance positive fluorescence. Further details are available in the below publication.

To minimize silencing it is recommended to propagate on paromycin and hygromycin.

Itakura AK, Chan KX, Atkinson N, Pallesen L, Wang L, Reeves G, Patena W, Caspari O, Roth R, Goodenough U, McCormick AJ, Griffiths H, Jonikas MC. A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18445-18454. doi: 10.1073/pnas.1904587116. Epub 2019 Aug 27. PMID: 31455733; PMCID: PMC6744930.

CC-5422 saga1::SAGA1-Venus3xFLAG

Products

Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Cart