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CC-5499 ∆ChR1 ∆ChR2 [PH128]

Name: CC-5499 ∆ChR1 ∆ChR2 [PH128]
SKU: CC-5499 ∆ChR1 ∆ChR2 [PH128]
Availability: InStock

$30.00

Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, March 2019

This is a ChR1 /ChR2 disruption strain, generated with CRISPR/Cas9.

Background strain: CC-125
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (p114), pAPHVII (p360)
Target gene: ChR1 (COP3), Cre14.g611300; ChR2 (COP4), Cre02.g085257
Target sequence: ChR1: TGTGGCTTCGTTACGCGGAGTGG (Exon5); ChR2: GCTCGCGCCCAACGGCACTCAGG (Exon1)

Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains

Visit www.chlamy.de for more info or contact CRISPR@chlamy.de

This is an unpublished strain. Please contact ph@chlamy.de before using it.

Baidukova O, Oppermann J, Kelterborn S, Fernandez Lahore RG, Schumacher D, Evers H, Kamrani YY, Hegemann P. Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation. Nat Commun. 2022 Nov 25;13(1):7253. doi: 10.1038/s41467-022-35018-6. PMID: 36433995; PMCID: PMC9700795.

Locus:
ChR1, ChR2
Chromosome:
14, 2
Mutation Information:
Predicted Mutation Effect:

CC-5499 ∆ChR1 ∆ChR2 [PH128]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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