CC-5510 pf2-4::DRC4-crCherry mt- [1-4]
$30.00
From Mary Porter, University of Minnesota, March 2019
CC-4483 pf2-4 was co-transformed with pSI103 and a wild-type copy of the DRC4 gene containing a codon optimized Cherry tag at its carboxy-terminus, selected on paromomycin, and screened for recovery of wild-type motility. The pf2-4 mutation in the DRC4 gene on chromosome 6 was rescued.
Rupp G, Porter ME. A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol. 2003 Jul 7;162(1):47-57. doi: 10.1083/jcb.200303019. PMID: 12847082; PMCID: PMC2172716.
Saravanan S, Trischler D, Bower R, Porter M, Lechtreck K. In vivo imaging reveals independent intraflagellar transport of the nexin-dynein regulatory complex subunits DRC2 and DRC4. Mol Biol Cell. 2023 Feb 1;34(2):br2. doi: 10.1091/mbc.E22-11-0524. Epub 2023 Jan 4. PMID: 36598807; PMCID: PMC9930527.