* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pRAM118_SAGA1_Venus_3xFLAG
$30.00
From Jessica Hennacy, Martin Jonikas lab, Princeton University, August 2020
pRAM118 is derived from pLM005, but contains a hygromycin selectable marker in lieu of the paromomycin cassette. All other features remain the same.
Selection: ampicillin resistant in E. coli; hygromycin in Chlamydomonas
takura AK, Chan KX, Atkinson N, Pallesen L, Wang L, Reeves G, Patena W, Caspari O, Roth R, Goodenough U, McCormick AJ, Griffiths H, Jonikas MC. A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18445-18454. doi: 10.1073/pnas.1904587116. Epub 2019 Aug 27. PMID: 31455733; PMCID: PMC6744930.