CC-5662 labE mt+

$30.00

From Alfredo Kono, Martin Spalding lab, Iowa State University, July 2020

This double mutant is the product of lcia90 mt+ (CC‐5067, LCIA mutant) backcrossed five times with pmp1 21gr mt- (CC‐5375, LCIB mutant) and requires high CO2 concentrations for phototrophic growth.

Mutant alleles: LCIB, Cre10.g452800.t1.1, chromosome_10:4608866..4611901 forward; LCIA, NAR 1.2, Cre06.g309000.t1.1, chromosome_6:8604782..8607670 reverse

Kono A, Spalding MH. LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO2 uptake under low CO2. Plant J. 2020 Jun;102(6):1127-1141. doi: 10.1111/tpj.14761. Epub 2020 Apr 27. PMID: 32248584.

Wang Y, Spalding MH. Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii. Plant Physiol. 2014 Dec;166(4):2040-50. doi: 10.1104/pp.114.248294. Epub 2014 Oct 21. PMID: 25336519; PMCID: PMC4256846.

  • Locus:
  • LCIB, LCIA/NAR 1.2
  • Chromosome:
  • 10,6
  • Mutation Information:
  • Predicted Mutation Effect:

Kono A, Spalding MH. LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO2 uptake under low CO2. Plant J. 2020 Jun;102(6):1127-1141. doi: 10.1111/tpj.14761. Epub 2020 Apr 27. PMID: 32248584.

Wang Y, Spalding MH. Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii. Plant Physiol. 2014 Dec;166(4):2040-50. doi: 10.1104/pp.114.248294. Epub 2014 Oct 21. PMID: 25336519; PMCID: PMC4256846.