* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pLM154: pLM099-Cre09.g394621-Venus-3xFLAG
$30.00
From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020
SAGA2 (Cre09.g394621) coding sequence plus 2216 bp upstream of the start codon was cloned into pLM099 by recombineering to produce a C-terminal CrVenus-3xFLAG tag. Transformed into C. reinhardtii strain CMJ030 to produce CC-5655.
Host strain: DH10B
Selection in E. coli: kanamycin
Selection in C. reinhardtii: paromomycin
Meyer MT, Itakura AK, Patena W, Wang L, He S, Emrich-Mills T, Lau CS, Yates G, Mackinder LCM, Jonikas MC. Assembly of the algal CO2-fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif. Sci Adv. 2020 Nov 11;6(46):eabd2408. doi: 10.1126/sciadv.abd2408. PMID: 33177094; PMCID: PMC7673724.