pRMC2
$30.00
From Anthony Berndt, Stephen Mayfield Lab, University of California, San Diego, November 2021
Source: Anthony Berndt, Stephen Mayfield Lab, University of California, San Diego
Plasmid: pRMC2
Vector: pUC19-based backbone
Host strain: DH5alpha
Origin: Gibson assembly using pUC19 backbone from synthesized gBlocks and elements of the pOpt vectors from the Kruse Lab.
Insert: Secretion directed SARS-CoV-2 Spike RBD fused to mClover fluorescent protein.
Selection in E. coli: Ampicillin
Comment: Transformed in to algae strain RMC2. pRMC2 map in GenBank format is also available in publication’s supplemental data.
Berndt AJ, Smalley TN, Ren B, Simkovsky R, Badary A, Sproles AE, Fields FJ, Torres-Tiji Y, Heredia V, Mayfield SP. Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii. PLoS One. 2021 Nov 18;16(11):e0257089. doi: 10.1371/journal.pone.0257089. PMID: 34793485; PMCID: PMC8601568.