* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5855 lcla1(LCLA1-3xFLAG) mt-

$30.00

From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022

Alias: lcla1#PH25-1 (pADC10)#25
Genotype: mt-; Cre07.g329861::APHVII; LCLA1-3xFLAG::ARG7
Background: CC-4533/CC-5155
Origin: lcla1#PH25-1 complemented strain, transformed with pADC10 (pADC2 with sequence encoding 3xFLAG epitope inserted at 3’ end of gene) for detection of LCLA1-3xFLAG. lcla1#PH25-1 (an arg7-8 spore obtained from the same cross as lcla1#PH25-6, that is, the 4th backcross of amc12#6E9 to CC-4533/CC-5155). The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.

CC-5855 lcla1(LCLA1-3xFLAG) mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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