CC-5859 armc2::ARMC2-mS ida3::IDA3-NG mt-
$30.00
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating armc2::ARMC2-mScarlet and ida3::IDA3-mNG. It expresses ARMC2-mScarlet, a cargo adapter for IFT of radial spokes, and IDA3-mNG, a cargo adapter for IFT of IDA I1/f, in a corresponding double mutant back ground. Very dim in full-length flagella but stronger during ciliary assembly.
Lechtreck KF, Liu Y, Dai J, Alkhofash RA, Butler J, Alford L, Yang P. Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes. Elife. 2022 Jan 4;11:e74993. doi: 10.7554/eLife.74993. PMID: 34982025; PMCID: PMC8789290.
Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS. The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell. 2018 Apr 15;29(8):886-896. doi: 10.1091/mbc.E17-12-0729. Epub 2018 Mar 29. PMID: 29467251; PMCID: PMC5896928.