CC-5866 gun4-II [6F14]
$30.00
From Mautusi Mitra, University of West Georgia, March 2022
Background: 4A+
Origin: random insertional mutagenesis using the linearized pBC1 plasmid which contains the AphVIII gene that confers resistance against paromomycin; pBC1 plasmid was obtained from Dr. Kris Niyogi (UC Berkeley); strain is paromomycin resistant; for details please see published research article
Culture maintenance: strain can be maintained under dim light (15-25 µmole m-2s-1); can be maintained on TAP or on TAP + Paromomycin plates containing 10 – 15 µg of Paromomycin/mL of TAP medium.
Comment: progressively photo-bleaches with increase in light intensity; see published article for more detail.
Grovenstein PB, Wilson DA, Lankford KD, Gaston KA, Perera S, Mitra M. Identification and molecular characterization of the second Chlamydomonas gun4 mutant, gun4-II. F1000Res. 2013 Jun 19;2:142. doi: 10.12688/f1000research.2-142.v2. PMID: 24627785; PMCID: PMC3931455.