* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pGEM-T-ARMC2–3XTAG–Hyg
$30.00
From Pinfen Yang, Marquette University, August 2022
Host strain: E. coli: DH5α
For plasmid stability, the bacteria should not be cultured for an excessive long duration. For liquid cultures, it is recommended to grow around room temperature for ~ 30 hours under the selection of carbenicillin.
Lechtreck KF, Liu Y, Dai J, Alkhofash RA, Butler J, Alford L, Yang P. Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes. Elife. 2022 Jan 4;11:e74993. doi: 10.7554/eLife.74993. PMID: 34982025; PMCID: PMC8789290.