* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5986 NAP1L1-mNeonGreen-3xFLAG-H10 mt+
$30.00
From George Witman, University of Massachusetts Medical School, January 2023
Background: CC-5415 nit1 agg1 mt+ [Witman g1]
Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.
Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.
Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.