* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6015 IFT46-mScarletI (no resistance markers)

Name: CC-6015 IFT46-mScarletI (no resistance markers)
SKU: CC-6015
Availability: InStock

$30.00

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124/CC-125

Origin: Back-cross of CC-6014 to CC-125 to restore wild-type DRC4 function and remove the AphVIII resistance marker previously used for selection

Proof of concept strain for cotargeted genome editing with the possibility of crossing out the resistance marker. See CC-6014 for original CRISPR strain with disrupted DRC4 gene. No antibiotic resistance present.

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.

Locus:
IFT46 (Cre05.g241637_4532)
Chromosome:
5
Mutation Information:
Predicted Mutation Effect:

CC-6015 IFT46-mScarletI (no resistance markers)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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