CC-6055 psbH::aadA knockout [HT72]

$30.00

From Saul Purton, University College London, August 2023

Origin: psbH::aadA knockout in which part of the psbH coding region and 3’ UTR is replaced with the aadA cassette through chloroplast transformation

Culture maintenance: Acetate-requiring, light-sensitive (ΔPSII mutant)

This strain was created to serve as a recipient for ‘marker-free’ chloroplast transformation where selection is based on restoration of phototrophy using the WT psbH gene as the marker. This strain has the same psbH::aadA genotype as strain TN72 (see: CC-5168) but was created using the cell-walled wild-type strain CC-1690 rather that a cell-wall deficient strain carrying the cw15 nuclear lesion. HT72 is therefore suitable for transformation using the microparticle bombardment method. 

Wannathong T, Waterhouse JC, Young RE, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Appl Microbiol Biotechnol. 2016 Jun;100(12):5467-77. doi: 10.1007/s00253-016-7354-6. Epub 2016 Feb 18. PMID: 26887319; PMCID: PMC4875957.

Larrea-Alvarez M, Young R, Purton S. A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii. Methods Mol Biol. 2021;2317:293-304. doi: 10.1007/978-1-0716-1472-3_17. PMID: 34028777.