* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pOpt3_Ppsad_5’psad_CTPpsadi1_aadA_strepII_Tfdx1_aadA_Min
$30.00
From Kyle Lauersen, King Abdullah University of Science and Technology (KAUST), Thuwal, KSA, August 2022
Host strain: DH5-α
Selection in E. coli: Ampicillin (100 µg/mL)
Reporter: Selection as reporter
Subcellular localization: Chloroplast (PsaD CTP)
Chlamydomonas Antibiotic selection: Spectinomycin – only one cassette (200 µg/mL)
SSB lab identifier: MS002
Gutiérrez S, Wellman GB, Lauersen KJ. Teaching an old 'doc' new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection. Front Bioeng Biotechnol. 2022 Sep 23;10:979607. doi: 10.3389/fbioe.2022.979607. PMID: 36213064; PMCID: PMC9540369.