* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pLM1392:L(-1)-TurboID
$30.00
From Chun Sing Lau, Luke Mackinder Lab, University of York, June 2023.
Host strain: DH5α
Selection in E. coli: Ampicillin
The codon-optimised TurboID sequence is synthesised by Twist Bioscience and cloned into the pTwistAmp High Copy backbone. This plasmid is used as a Level (-1) part to clone the target gene and TurboID tag into the pUAP1 backbone as a CDS part occupying the B3 – B4 position.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.