* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pLM547:pLM099-Cre09.g394510-Venus-3xFlag

$30.00

From Chun Sing Lau, Luke Mackinder Lab, University of York-UK, June 2023

Host strain: DH10B
Selection in E.coli: Kanamycin
Selection in C.reinhardtii: Paromomycin

Cre09.g394510 coding sequence plus 2142 bp upstream of the start codon was cloned into pLM099 by recombineering to produce a C-terminal CrVenus-3xFLAG tag.

Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.

pLM547:pLM099-Cre09.g394510-Venus-3xFlag

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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