CC-45 ac29 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate, somewhat chlorophyll deficient
From R.P. Levine, brought to Duke University by N.W. Gillham, 1968
This strain appears yellow-green in color, especially on minimal medium. The color difference from wild type is rather subtle. The AC29 locus is closely linked to mating type. This allele, ac29, is linked to mt+, whereas ac29a (CC-44) is linked to mt-. For an ac29 strain whose phenotype is easier to score, see CC-3054 ac29-3, a deletion mutant isolated by Patrick Ferris.
The AC29 locus encodes a protein that is a homolog of the plant protein ALBINO3, which is required for the integration of the light-harvesting complex into the thylakoid membrane.
Ferris PJ (1995) Localization of the nic-7, ac-29 and thi-10 genes within the mating-type locus of Chlamydomonas reinhardtii. Genetics 141:543-549
Ferris PJ, Armbrust EV, Goodenough UW (2002) Genetic structure of the mating-type locus of Chlamydomonas reinhardtii. Genetics 160:181-200
Bellafiore S, Ferris P, Naver H, Göhre V, Rochaix JD (2002) Loss of Albino3 leads to the specific depletion of the light-harvesting system. Plant Cell 14:2303-2314