CC-48 arg2 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: requires arginine
CC-48 came from R.P. Levine, and was brought to Duke University by N.W. Gillham in 1968. This is a stable arginine-requiring strain, easy to score.
The arg2 mutation, which was UV-induced by Eversole, is an allele at the ARG7 locus, encoding argininosuccinate lyase, and is also known as arg7-8. These two mutations are capable of intragenic complementation, and are often used to create diploid strains. Diploids formed between arg2 and the original arg7 mutant are stable and grow at wild type rates in the absence of arginine. Intragenic complementation is also seen between arg2 and arg7-2, arg7-3, arg7-4, arg7-5, arg7-7, and arg7-12 (see Loppes et al., 1972, and Matagne, 1978).
The wild type gene at this locus was cloned by Debuchy et al., and used to transform arg7 mutants to arginine independence.
The arg2 and arg7 mutants require arginine (50 micrograms per ml is recommended) and cannot use ornithine or citrulline. They also grow well on medium containing yeast extract (4 grams per liter).
The following references pertain to arg2 and to other alleles at the ARG7 locus:
Eversole RA (1956) Biochemical Mutants of Chlamydomonas reinhardi. Amer J Bot 43:404-407
Ebersold WT, Levine RP (1959) A genetic analysis of linkage group I of Chlamydomonas reinhardi. Z Vererbungsl. Z Vererbs 90:74-82
Hudock GS (1962) The pathway of arginine biosynthesis in Chlamydomonas reinhardi. Biochem. Biophys Res Commun 9:551-555
Hudock GA (1963) Repression of arginosuccinase in Chlamydomonas reinhardi. Biochem. Biophys. Res Commun 10:133-138
Gillham NW (1965) Induction of chromosomal and nonchromosomal mutations in Chlamydomonas reinhardi with N-methyl-N'-nitro-N-nitrosoguanidine. Genetics 52:529-537
Loppes R (1966) Damage induced by methyl methanesulfonate (MMS) in Chlamydomonas reinhardi. Z. Vererbs. 98:193-202
Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol. Gen. Genet. 104:172-177
Strijkert PJ and Sussenbach JS (1969) Arginine metabolism in Chlamydomonas reinhardi. Evidence for a specific regulatory mechanism of the biosynthesis. Eur J Biochem 8:408-412
Sussenbach JS and Strijkert PJ (1969) Arginine metabolism in Chlamydomonas reinhardi. On the regulation of the arginine biosynthesis. Eur J Biochem 8:403-407
Loppes R (1970) Selection of arginine-requiring mutants in Chlamydomonas reinhardi after treatment with 3 mutagens. Experientia 26:660-661
Levine RP and Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408
Loppes R and Matagne RF (1972) Allelic Complementation between arg-7 mutants in Chlamydomonas reinhardi. Genetica 43:422-430
Loppes R and Strijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257
Loppes R, Matagne RF, Strijkert PJ (1972) Complementation at ARG-7 locus in Chlamydomonas reinhardi. Heredity 28:239-251
McMahon D and Langstroth P (1972) The effects of canavanine and of arginine starvation on macromolecular synthesis in Chlamydomonas reinhardi. J Gen Microbiol 73:239-250
Strijkert PJ, Loppes R, Sussenbach JS (1973) The actual biochemical block in the arg-2 mutant of Chlamydomonas reinhardi. Biochem Genet 8:239-248
Konvalinkova V, Matagne RF, Loppes R (1974) Induction and analysis of revertants
from various arg-7 mutants lacking argininosuccinate lyase in Chlamydomonas
reinhardi. Mut Res 24:69-72
Schimmer R and Loppes R (1975) Forward mutations induced by nitrosoguanidine during the synchronized cell-cycle of Chlamydomonas reinhardtii. Mol Gen Genet 138:25-31
Matagne RF (1976) Arg-7 mutant X wild-type crosses in Chlamydomonas reinhardi: study of the enzyme produced in diploid strains. Mol Gen Genet 146:209-214
Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99
Matagne RF and Vincenzotto C (1979) Study of the argininosuccinate lyase produced by triallelic complementation in triploids of Chlamydomonas reinhardi [proceedings]. Arch. Int Physiol Biochim 87:832-833
Debuchy R, Purton S, Rochaix JD (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J 8:2803-2809
Gumpel NJ, Purton S (1994) Playing tag with Chlamydomonas. Trends Cell Biol 4:299-301