CC-1015 ac-u-c-2-43 (atpB deletion) mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate
This mutation consists of deletion of one entire rRNA region plus the adjoining unique sequence region of Eco 11/Bam 10, producing an atpB-deficient phenotype. The chloroplast ribosomes appear normal.
A fusion fragment was sequenced by Scott Newman in 1989, unpublished. The mutation appears to have arisen by illegitimate recombination between dispersed short repeat segments flanking the atpB and 5S rRNA genes.
Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190