CC-1021 wild type mt+ [Spreitzer and Mets 2137]

$30.00

From Robert Hodson, University of Delaware, April 1980

Strain 2137 was isolated by Robert Spreitzer in the laboratory of Laurie Mets, from a cross of Sager’s 21 gr strain (equivalent to CC-1690) to 137c mt- (equivalent to CC-124). A product was selected for ability to grow as single cells in minimal medium, for negative phototaxis (agg1) and for green color when grown in the dark. Subsequently many mutants have been isolated in this background.

Please see CC-3269 for another 2137 stock, obtained directly from Laurie Mets. Unless you have already used CC-1021 and want the identical strain for comparison, please order CC-3269.

This strain was originally reported to contain nit1 but not nit2 based on crosses done by the Chlamydomonas Genetics Center in the early 1980s. However, Olivier Vallon has found that it does not grow on nitrite medium, suggesting a nit2 mutation (pers. comm. August 1995). A cross to wild type nit+ carried out by the Chlamydomonas Genetics Center in September 1995 confirmed that this isolate does contain two nit mutations.

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569