CC-1024 pf1 mt+
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained his pf1 strain from Warr, which makes us suspect that this is one of the two pf1 alleles isolated by McVittie rather than the original pf1 isolated by Lewin and subsequently characterized in Levine’s laboratory (see CC-602). This strain is derivative pf1.31D+ from Luck’s lab.
Cells of the pf1 mutant lack flagellar spokeheads. Motility is impaired, and the phenotype is easy to score. The PF1 locus encodes flagellar radial spoke protein 4 (RSP4), a component of the radial spoke head.
McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM, Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB (2006) Radial spoke proteins of Chlamydomonas flagella. J Cell Sci 119(Pt 6):1165-1174
McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM, Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB (2006) Radial spoke proteins of Chlamydomonas flagella. J Cell Sci 119(Pt 6):1165-1174