CC-1028 pf5 mt+
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s notes state that he obtained this mutant from Chua who got it from Levine. This stock is derivative pf5.6B+. An allelic mutant (pf5-A of Huang et al., 1981), was isolated in Luck’s laboratory. Huang et al. reported that their two pf5 mutants lacked four flagellar polypeptides: radial spoke components 13 and 15, component 1 of the innter dynein arms, and a low molecular weight axonemal protein whose structural localization was unknown.
Working with CC-1331, which was also originally designated pf5, Yang et al. (2009) determined that the mutation in that strain was in the FLA14 gene encoding the dynein light chain LC8, and renamed pf5 as fla14-3. They reported however that CC-1028 had a wild-type FLA14 gene, capable of rescuing the CC-1331 phenotype.
Since genetic analysis at the Chlamydomonas Genetics Center at Duke confirmed the expected linkage of the pf5 mutation in CC-1028 and its derivatives to linkage group III, we conclude that the mutation in CC-1028 and the one in CC-1331 are in different genes, but probably closely linked on the same chromosome.
Huang B, Piperno G, Ramanis Z, Luck DJ (1981) Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function. J Cell Biol 88:80-88
Yang P, Yang C, Wirschell M, Davis S (2009) Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes. J Biol Chem 284:31412-31421
Huang B, Piperno G, Ramanis Z, Luck DJ (1981) Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function. J Cell Biol 88:80-88
Yang P, Yang C, Wirschell M, Davis S (2009) Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes. J Biol Chem 284:31412-31421