CC-1029 pf6 mt+
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained this mutant from Chua who got it from Levine. This stock is derivative pf6.4D+.
Cells of this mutant ijiggle but cannot swim. Wargo et al. determined that it lacks the C1a projection on the central pair microtubules, and that it is also defective in calcium-induced modulation of dynein activity on specific doublet microtubules.
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
Rupp G, O'Toole E, Porter ME (2001) The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility. Mol Biol Cell 12:739-751
Wargo MJ, Dymek EE, Smith EF (2005) Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus. J Cell Sci 118:4655-4665
Zhang Z, Jones BH, Tang W, Moss SB, Wei Z, Ho C, Pollack M, Horowitz E, Bennett J, Baker ME, Strauss JF 3rd (2005) Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16. Mol Cell Proteomics 4:914-923
Boesger J, Wagner V, Weisheit W, Mittag M (2009) Analysis of flagellar phosphoproteins from Chlamydomonas reinhardtii. Eukaryotic Cell 8:922-932
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
Rupp G, O'Toole E, Porter ME (2001) The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility. Mol Biol Cell 12:739-751
Wargo MJ, Dymek EE, Smith EF (2005) Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus. J Cell Sci 118:4655-4665
Zhang Z, Jones BH, Tang W, Moss SB, Wei Z, Ho C, Pollack M, Horowitz E, Bennett J, Baker ME, Strauss JF 3rd (2005) Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16. Mol Cell Proteomics 4:914-923
Boesger J, Wagner V, Weisheit W, Mittag M (2009) Analysis of flagellar phosphoproteins from Chlamydomonas reinhardtii. Eukaryotic Cell 8:922-932