CC-104 spr-u-1-27-3 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (spectinomycin)
CC-104 was derived from a cross of CC-105 spr-u-1-27-3 mt+ to an ac20 cr1 strain. A tetratype tetrad was selected; this is the product that carries the wild type alleles of the ac20 and cr1 mutations.
Because this strain was used frequently in chloroplast genetics studies in the Boynton-Gillham laboratory, it was chosen from among several spr-u-1-27-3 strains as the representative mt+ stock for that mutation in the core collection.
spr-u-1-27-3 is a G -> A mutation at bp 1125 in the chloroplast 16S rRNA gene, and confers relatively low levels of spectinomycin resistance. Cells of this mutant grown on minimal medium on agar are killed by 100 micrograms/ml spectinomycin but will grow on acetate at this concentration.
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292