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CC-1085 nit1 mt+

Name: CC-1085 nit1 mt+
SKU: CC-1085
Availability: InStock

$30.00

Chlamydomonas Genetics Center, Duke University, 1980

From a cross of CC-1039 wild type nit+ mt+ [Sager’s 21 gr, stock subsequently replaced by CC-1690] x CC-1064 nit1 msr1 ac17 sr-u-sm2 mt- [a stock no longer in the collection].

This is the nit1-137 allele, which arose spontaneously in the 137c wild type background, and renders cells deficient in nitrate reductase, requiring a reduced nitrogen source (e.g. ammonium) for growth. Most 137c strains also carry the nit2-137 mutation, but CC-1085 has the wild type allele at the NIT2 locus.

This mutant is rescued by pMN24.

Fernández E and Matagne RF (1984) Genetic analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardii. Curr Genet 8:635-640

Fernández E and Matagne RF (1986) In vivo complementation analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardtii. Curr Genet 10:397-403

Locus:
NIT1
Chromosome:
9

CC-1085 nit1 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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