* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-110 spr-u-1-6-2 mt+

Name: CC-110 spr-u-1-6-2 mt+
SKU: CC-110
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University

Phenotype: antibiotic resistant (spectinomycin)

This strain was derived at Duke from a cross between CC-147 nr1-8 spr-u-1-62 and CC-198 er-u-37 sr-u-2-60; it does not carry nr1-8 or the markers from CC-198.

This is a good chloroplast resistance marker, easy to score on 100 microgram/ml spectinomycin. spr-u-1-6-2 is an A to G mutation at position 1123 in the chloroplast 16S rRNA and confers high level spectinomycin resistance under all growth conditions.

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292

Locus:
rrnS
Chromosome:
chloroplast

CC-110 spr-u-1-6-2 mt+

Products

Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Cart