CC-112 sr1 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (streptomycin)
Originally from R.P. Levine, designated sr-1-H-5; this stock is a derivative of the mutant strain originally isolated by N.W. Gillham in Levine’s laboratory and was designated sr-1a. Although alleles at the SR1 locus are readily obtained, and are mentioned in papers by Sager and others, we think the sr1 mutation in the various multiply marked strains in our collection is probably this one.
This is an excellent marker for linkage group IX, easy to score as resistant to 100 micrograms/ml streptomycin. In contrast to some chloroplast-encoded streptomycin-resistant mutants, sr1 is not resistant to 500 micrograms/ml streptomcin.
Bartlett et al. showed that this mutation confers resistance directly on the small subunit of the chloroplast ribosome. Based on its map position and analogous mutations in bacteria, we suspect that the SR1 locus may correspond to the PRPS17 gene, encoding protein S17 of the chloroplast ribosome.
Sager R (1955) Inheritance in the Green Alga Chlamydomonas Reinhardi. Genetics 40:476-489
Gillham NW and Levine RP (1962a) Studies on the origin of streptomycin resistant mutants in Chlamydomonas reinhardi. Genetics 47:1463-1474
Bartlett SG, Harris EH, Grabowy CT, Gillham NW, Boynton JE (1979) Ribosomal subunits affected by antibiotic resistance mutations at seven chloroplast loci in Chlamydomonas reinhardtii. Mol Gen Genet 176:199-208