* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-1144 sr1 msr1 nr1 act2 pyr1 NIT+ mt-
$30.00
Chlamydomonas Genetics Center, Duke University, February 1981
Phenotype: resistant to antibiotics and metabolic inhibitors (cycloheximide, methionine sulfoximine, neamine, pyrithiamine, and streptomycin)
From a cross of CC-975, a multiply marked strain, to CC-919, a wild type strain with nitrate reductase activity.
This was created as a multiply marked strain in a nit+ background, free of auxotrophic and pf markers. Although CC-1144 swims normally, some progeny in crosses seem to have impaired motility, suggesting that it may actually contain pf2 from the CC-975 parent strain, plus a segregating suppressor. CC-1144 carries the wild type alleles at both the NIT1 and NIT2 loci, and can therefore grow on nitrate medium.