CC-1154 me1 mt+
$30.00
From Paul Bolen in Boynton-Gillham laboratory, Duke University, May 1981
From a cross by Paul Bolen in the Boynton/Gillham lab, CC-125 wild type mt+ x CC-1153 sr-u-sm2 spr-u-sp-23 er? me1.
This strain carries the me1 mutation which constitutively methylates chloroplast DNA; described by Bolen et al. Cell 28, 335-343 (1982). It may also carry a nuclear erythromycin resistance mutation, allelism of which has not be tested.
Bolen PL, Grant DM, Swinton D, Boynton JE, Gillham NW (1982) Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloroplast gene transmission in chlamydomonas. Cell 28:335-343
Sager R, Grabowy C (1983) Differential methylation of chloroplast DNA regulates maternal inheritance in a methylated mutant of Chlamydomonas. Proc Natl Acad Sci USA 80:3025-3029
Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H (2002) A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes. Proc Natl Acad Sci USA 99:5925-5930