* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-118 sr-u-2-60 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (streptomycin)
CC-118 was derived from a cross of wild type mt+ x sr-u-2-60 mt- (equivalent to CC-119).
This is an excellent chloroplast mutant, easy to score as resistant to up to 500 micrograms/ml streptomycin on agar (50-100 micrograms is sufficient to kill wild type cells). The mutation is an A to C change at base 474 of 16S rRNA gene (equivalent to E. coli 523).
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292