CC-1254 pf9 mt+
$30.00
Chlamydomonas Genetics Center, Duke University, 1981
Phenotype: impaired motility
From a cross CC-1090 fsr1 mt+ x CC-18 pf9 mt-. This isolate was selected for scorable pf phenotype. It is sensitive to fusidic acid.
This pf9 allele (= pf9-1) was isolated in Levine’s laboratory by UV irradiation of thi8 mt+. The mutant was originally described as sluggish. Subsequent analysis has shown that pf9 mutants swim with nearly normal flagellar beat frequency but decreased velocity owing to reduction in the amplitude of the flagellar waveform
Porter et al. showed that the pf9 mutants have inner arm dynein defects, and Myster et al. established that the mutations are in the 1-alpha dynein heavy chain DHC1.
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Porter ME, Power J, Dutcher SK (1992) Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms. J Cell Biol 118:1163-1176
Myster SH, Knott JA, Wysocki KM, O'Toole E, Porter ME (1999) Domains in the 1alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas. J Cell Biol 146:801-818