CC-1289 FUD17 (atpE) mt-
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, January 1982
Phenotype: requires acetate
This mutant was isolated by Bennoun et al. after metronidazole treatment. Lemaire and Wollman reported a specific deficiency in synthesis of the epsilon subunit of ATP synthase, and Robertson et al. showed that the mutation is a frameshift in the atpE gene
[Please note that while this paper is correct on the FUD17 mutation, the report that rps7 was a split gene was erroneous.]
Bennoun P, Masson A, Delosme M (1980) A method for complementation analysis of nuclear and chloroplast mutants of photosynthesis in chlamydomonas. Genetics 95:39-47
Lemaire C, Wollman FA (1989) The chloroplast ATP synthase in Chlamydomonas reinhardtii. II. Biochemical studies on its biogenesis using mutants defective in photophosphorylation. J Biol Chem 264:10235-10242
Robertson D, Boynton JE, Gillham NW (1990) Cotranscription of the wild-type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3' half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE. Mol Gen Genet 221:155-163