CC-1328 agg1 mt+
$30.00
From Robert Smyth, June 1982
Phenotype: altered phototaxis
The agg1 allele is a naturally occurring variant found in the wild type strain CC-124 and was described as as np (negatively phototactic) by Smith and Ebersold. agg1 cells show strong negative phototaxis, and form a tight pellet at the bottom of a liquid culture in overhead light.
Since it occurs in a wild type strain that has been involved in many crosses over the years, the agg1 allele is probably widespread throughout strains in the Chlamydomonas Resource Center collection. However, most cultures in the collection have not been tested for this phenotype.
CC-1328 is a clean agg1 mutation in mating type plus.
Hudock GA, Hudock MO (1973) Phototaxis: isolation of mutant strains of Chlamydomonas reinhardi with reversed sign of response. J Protozool 20:139-140
Smyth RD, Martinek GW, Ebersold WT (1975) Linkage of six genes in Chlamydomonas reinhardtii and the construction of linkage test strains. J Bacteriol 124:1615-1617
Smyth RD, Ebersold WT (1985) Genetic investigation of a negatively phototactic strain of Chlamydomonas reinhardtii. Genet Res 46:133-148
Zacks DN, Spudich JL (1994) Gain setting in Chlamydomonas reinhardtii: mechanism of phototaxis and the role of the photophobic response. Cell Motil Cytoskeleton 29:225-230