* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-1370 fa1 mt+

Name: CC-1370 fa1 mt+
SKU: CC-1370
Availability: InStock

$30.00

From Ralph Lewin, Scripps Institution of Oceanography, October 1982

Phenotype: impaired flagellar autotomy

The fa1 mutant was isolated by Lewin and Burrascano after UV mutagenesis, and characterized as a mutant that does not shed its flagella under conditions such as pH shock, treatment with ethanol, etc. Further analysis by Finst et al. showed that the defect is specifically in severing outer doublets of the microtubules in response to calcium, and that the corresponding gene is a novel 171-kD protein.

Lewin R and Burrascano C (1983) Another new kind of Chlamydomonas mutant, with impaired flagellar autotomy. Experientia 39:1397-1398

Quarmby LM (1996) Ca2+ influx activated by low pH in Chlamydomonas. J Gen Physiol 108:351-361

Evans JH and Keller LR (1997) Receptor-Mediated Calcium Influx in Chlamydomonas reinhardtii. J. Euk Microbiol 44:237-245

Yueh YG, Crain RC (1993) Deflagellation of Chlamydomonas reinhardtii follows a rapid transitory accumulation of inositol 1,4,5-trisphosphate and requires Ca2+ entry. J Cell Biol 123:869-875

Cheshire JL, Evans JH, Keller LR (1994) Ca2+ signaling in the Chlamydomonas flagellar regeneration system: cellular and molecular responses. J Cell Sci 107:2491-2498

Crain RC, Yueh YG (1995) Phosphoinositide signalling in plant and algal responses to physiological stimuli. Biochem Soc Trans 23:853-856

Hayashi M, Yagi T, Yoshimura K, Kamiya R (1998) Real-time observation of Ca2+-induced basal body reorientation in Chlamydomonas. Cell Motil Cytoskeleton 41:49-56

Tuxhorn J, Daise T, Dentler WL (1998) Regulation of flagellar length in Chlamydomonas. Cell Motil Cytoskeleton 40:133-146

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

Finst RJ, Kim PJ, Griffis ER, Quarmby LM (2000) Fa1p is a 171 kDa protein essential for axonemal microtubule severing in Chlamydomonas. J Cell Sci 113:1963-1971

Hayashi M, Yanagisawa HA, Hirono M, Kamiya R (2002) Rescue of a Chlamydomonas inner-arm-dynein-deficient mutant by electroporation-mediated delivery of recombinant p28 light chain. Cell Motil Cytoskeleton 53:273-280

Locus:
FA1
Chromosome:
6

CC-1370 fa1 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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