CC-1375 ac-u-lambda (psbA deletion) mt+
$30.00
Boynton-Gillham laboratory, Duke University, 1982
Phenotype: requires acetate
This strain is a mt+ derivative, obtained by successive crosses to CC-125 wild type of a mutant isolated in a diploid strain. This strain is not arginine-requiring. The mutant shows symmetrical 9 kb deletions in both copies of the psbA gene.
This mutant was isolated in an arg2/arg7 diploid strain by Doug Rabert in 1979. Cells were pregrown in 0.5 mM 5-fluorodeoxyuridine in liquid cultures, concentrated, and irradiated with 2 minutes X-ray @ 6 krad/min. The irradiated cells were plated on 2 mM arsenate and arsenate-tolerant (non-photosynthesizing) clones were selected.
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151