CC-1387 pf27 mt+
$30.00
From David Luck, Rockefeller University, February 1983, his isolation number ncs-83
Phenotype: impaired motility
In pf27 cells, several polypeptides associated with radial spokes are not phosphorylated, but dikaryon rescue experiments suggest that the product of the PF27 locus is not actually a radial spoke component.
This mutant is non-motile and easy to score, and is a good marker for the centromere of linkage group XII/XIII.
Huang B, Piperno G, Ramanis Z, Luck DJ (1981) Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function. J Cell Biol 88:80-88
Curry AM, Rosenbaum JL (1993) Flagellar radial spoke: a model molecular genetic system for studying organelle assembly. Cell Motil Cytoskeleton 24:224-232
Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99
Yang C, Yang P (2006) The flagellar motility of Chlamydomonas pf25 mutant lacking an AKAP-binding protein is overtly sensitive to medium conditions. Mol Biol Cell 17:227-238