CC-1398 sup-pf2 mt-
$30.00
From David Luck, Rockefeller University, February 1983 his isolation number ncs-3R4
The sup-pf2 mutant was isolated in a pf24 strain by Huang et al., and was originally characterized as a mutation in the spoke specific suppressor system, which restores motility to radial spoke- and central-pair defective mutants without altering spoke or central pair defects. It was subsequently shown to be an allele at the ODA2 locus, corresponding to the gene encoding the gamma heavy chain of the outer dynein arm.
To the best of our knowledge, this is the original sup-pf2 allele.
Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-124
Brokaw CJ, Luck DJ, Huang B (1982) Analysis of the movement of Chlamydomonas flagella:" the function of the radial-spoke system is revealed by comparison of wild-type and mutant flagella. J Cell Biol 92:722-732
Rupp G, O'Toole E, Gardner LC, Mitchell BF, Porter ME (1996) The sup-pf-2 mutations of Chlamydomonas alter the activity of the outer dynein arms by modification of the gamma-dynein heavy chain. J Cell Biol 135:1853-1865
Mitchell BF, Grulich LE, Mader MM (2004) Flagellar quiescence in Chlamydomonas: Characterization and defective quiescence in cells carrying sup-pf-1 and sup-pf-2 outer dynein arm mutations. Cell Motil Cytoskeleton 57:186-196